BPC-157 + TB-500 — Research Blend (Barrier Peptide + Thymosin-β4 Fragment)

This blended mix combines BPC-157 (a gastric “Body Protection Compound” fragment) with a research fragment of Thymosin-β4 (TB-500). It’s designed for laboratories studying cell migration, cytoskeleton dynamics, ECM remodeling, angiogenic signaling, and barrier integrity in tissue models.

Component A — BPC-157

CAS: 137525-51-0
Sequence (15 aa): GEPPPGKPADDAGLV
Notes: Synthetic fragment derived from a gastric protein; used to explore barrier support, angiogenic cues, and cell migration in vitro/in vivo models.

Component B — TB-500 (Thymosin-β4 fragment)

Parent protein: Thymosin-β4 (Tβ4, 43 aa)
Fragment: Research-grade Tβ4-derived sequence (TB-500); focuses on actin binding/motility and cell migration pathways.
Notes: Often studied for effects on cytoskeleton reorganization, angiogenesis markers, and matrix remodeling.

Blend Rationale (Plain English)

BPC-157 is frequently used for barrier/ECM context (endothelium, epithelium, tendon/ligament models) and signaling that supports migration & angiogenic cues.
TB-500 brings complementary actin-motility biology and cell-movement dynamics, useful in wound-closure and sprouting assays.
Together, the blend allows labs to test ECM + motility + angiogenic endpoints in a single protocol, with options to vary ratios for mechanism mapping.

Potential Research Applications

Cell Migration & Motility

Scratch (“wound-healing”) assays and live-cell tracking
Actin polymerization / focal adhesion markers

ECM & Barrier Integrity

Trans-epithelial electrical resistance (TEER)
Collagen I/III, laminin, and MMP/TIMP panels

Angiogenesis Panels

Tubulogenesis, sprouting, and VEGF-pathway readouts
Pericyte–endothelial co-culture systems

Blend Options (Research Configurations)

Standard

1:1 mass ratio (BPC:TB)
Balanced ECM + motility coverage

ECM-Forward

2:1 (BPC-heavy)
Emphasis on barrier metrics and collagen markers

Motility-Forward

1:2 (TB-heavy)
Emphasis on actin dynamics and migration speed

Design Notes

Pre-define primary endpoints (migration rate, TEER change, tubule length).
Specify salt form and end-groups (e.g., acetate, amidation) on each component in your methods.
Document vehicle (pH, ionic strength) — ECM and cytoskeletal readouts are condition-sensitive.

Key Study Themes (Educational Summary)

BPC-157 — barrier & ECM signaling

Frequently reported to influence endothelial/epithelial integrity and angiogenic cues in preclinical models.
Common endpoints: TEER, tight-junction proteins, collagen expression, and in vitro tubulogenesis.

TB-500 — actin & motility biology

Derived from Tβ4 research; used to study G-actin binding, cell motility, and sprouting in migration assays.
Common endpoints: wound-closure rate, actin staining, focal-adhesion proteins, and angiogenic sprout length.

Synergistic Peptides (for Study Design)

GHK-Cu

Why pair: ECM/trophic gene programs; complements collagen/repair markers.
Angle: Matrix + angiogenesis arrays with imaging of collagen deposition.

LL-37

Why pair: Epithelial defense and wound-closure context to round out barrier biology.
Angle: Co-culture wound models with cytokine readouts.

Tα1 (Thymosin-α1)

Why pair: Immune-context studies (innate→adaptive) alongside ECM/motility endpoints.
Angle: Cytokine panels + barrier integrity under challenge.

Controls

Include vehicle-only and single-agent arms to separate blend effects.
Add timing (co- vs post-challenge) and dose-response curves for mechanism clarity.

Known Concerns (Context)

Co-formulation: Verify compatibility (pH, buffer salts, preservatives) before blending; avoid unnecessary excipients that can confound cell assays.
Stability: Prepare fresh aliquots; minimize freeze–thaw cycles; protect from light/moisture.
Model variability: Migration/ECM endpoints can vary with serum lots, substrate coatings, and cell passage number — document conditions tightly.
General: For laboratory research use only; not for human consumption or therapeutic use.

Specifications & Handling

Form: Lyophilized powders blended to order (lot-coded)
Purity (each component): ≥ 99% (HPLC/MS verified)
Storage: ≤ −20 °C; dry, low-light conditions

In solution: Use sterile diluent; record pH/vehicle; aliquot immediately; avoid repeat freeze–thaw
Packaging: Tamper-evident; research-only labeling; blend ratio indicated on label

Regulatory & Use Notice

Sold for laboratory research use only. Not for human consumption, medical, or veterinary use. No human-use instructions are provided. Buyer is responsible for safe handling and regulatory compliance.